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Publications

Cancer Research

  1. Whitney S Henry, Sebastian Müller, Jia-Shu Yang, Sarah Innes-Gold, Sunny Das, Ferenc Reinhardt, Kim Sigmund, Vaishnavi V Phadnis, Zhengpeng Wan, Elinor Eaton, Julio L Sampaio, George W Bell, Amartya Viravalli, Paula T Hammond, Roger D Kamm, Adam E Cohen, Natalie Boehnke, Victor W Hsu, Kandice Ezra Levental, Raphael Rodriguez*, Robert A Weinberg*. “Ether lipids influence cancer cell fate by modulating iron uptake.”  bioRxiv 2024.03.20.585922. [Link] [PDF]

  2. Zhengpeng Wan#, Shun Zhang#, Amy X. Zhong, Liling Xu, Mark F. Coughlin, Georgios Pavlou, Sarah E. Shelton, Huu Tuan Nguyen, Satomi Hirose, Seunggyu Kim, Marie A. Floryan, David A. Barbie, F. Stephen Hodi*, Roger D. Kamm*. “Transmural Flow Upregulates PD-L1 Expression in Microvascular Networks.” Advanced Science, 2024, 2400921. [Link] [PDF]

  3. Huu Tuan Nguyen*, Ellen L Kan, Mouhita Humayun, Nadia Gurvich, Giovanni Offeddu, Zhengpeng Wan, Mark Coughlin, Diana Renteria, Andreas Loew, Susan Wilson, Christie Zhang, Vivian Vu, Sharon Wei Ling Lee, Seng-Lai Tan, David Barbie, Jonathan Hsu, Mark Gillrie*, Roger Kamm*. “Patient-Specific Vascularized Tumor Model: Blocking TAM Recruitment with Multispecific Antibodies Targeting CCR2 and CSF-1R.” Biomaterials, 2024, 122731. [Link] [PDF]

  4. Giovanni Offeddu, Elena Cambria, Sarah Shelton, Kristina Haase, Zhengpeng Wan, Luca Possenti, Huu Tuan Nguyen, Mark R Gillrie, Dean Hickman, Charles G Knutson*, Roger Kamm*. “Personalized Vascularized Models of Breast Cancer Desmoplasia Reveal Biomechanical Determinants of Drug Delivery to the Tumor.” Advanced Science, 2024, 2402757. [Link] [PDF]

  5. Emmanouil Angelidakis, Sophia Chen, Shun Zhang, Zhengpeng Wan, Roger D Kamm*, Sarah E Shelton*. “Impact of fibrinogen, fibrin thrombi and thrombin on cancer cell extravasation using in vitro microvascular networks.” Advanced Healthcare Materials, 2023, 202202984. [Link] [PDF]

  6. Zhengpeng Wan#, Marie A Floryan#, Mark F Coughlin, Shun Zhang, Amy X Zhong, Sarah E Shelton, Xun Wang, Chenguang Xu*, David A Barbie*, Roger D Kamm*. “New Strategy for Promoting Vascularization in Tumor Spheroids in a Microfluidic Assay.” Advanced Healthcare Materials, 2022, 2201784. [Link] [PDF]

  7. Seunggyu Kim#, Zhengpeng Wan#, Jessie S Jeon*, Roger D Kamm*. “Microfluidic Vascular Models of Tumor Cell Extravasation.” Frontiers in Oncology, 2022, 12:1052192. (Review) [Link] [PDF]

  8. Giovanni S Offeddu, Cynthia Hajal, Colleen R Foley, Zhengpeng Wan, Lina Ibrahim, Mark F Coughlin, Roger D Kamm*. “The cancer glycocalyx mediates intravascular adhesion and extravasation during metastatic dissemination.” Communications Biology, 2021, 4 (1), 1-10. [Link] [PDF]

  9. Zhengpeng Wan, Roger D Kamm*. “Microfluidic assessment of metastatic potential.” Nature Biomedical Engineering, 2019, 3 (8), 670-670. (Review) [Link] [PDF]

Vascularized Tissue Engineering

  1. Seunggyu Kim, Tianxin Cao, Zhengpeng Wan, Jaesang Kim, Zhuxuan Li, Legairre A. Radden II, Rakesh Santhanam, Eunkyung Clare Ko, Tatsuya Osaki, Sarah Spitz, Hyunmin Moon, Maria Proestaki, Seokbeom Roh, Gyudo Lee, Jessie S. Jeon, Curtis R. Warren, Roger D. Kamm. “A Microphysiological Interface of Skeletal Myobundles and Inflamed Adipose Tissue for Recapitulating Muscle Dysfunction in an Obese Microenvironment.” Advanced Healthcare Materials, (2025): e02711. [Link] [PDF]

  2. Marie Floryan, Elena Cambria, Adriana Blazeski, Mark F. Coughlin, Zhengpeng Wan, Giovanni Offeddu, Vinayak Vinayak, Aayush Kant, Jordan Whisler, Vivek Shenoy, Roger D. Kamm. “Long-term physiological flow rescues regressed microvascular networks and increases their longevity.” npj Biological Physics and Mechanics, 2, 24 (2025). [Link] [PDF]

  3. Alexsia Richards#, Andrew S Khalil#, Max Friesen, Troy W Whitfield, Xinlei Gao, Tenzin Lungjangwa, Roger D Kamm, Zhengpeng Wan, Lee Gehrke, David Mooney*, Rudolf Jaenisch*. “SARS-CoV-2 infection of human pluripotent stem cell-derived vascular cells reveals smooth muscle cells as key mediators of vascular pathology during infection.” Nature Communications, 15, 10754 (2024). [Link] [PDF]

  4. Kunzan Liu, Honghao Cao, Kasey Shashaty, Li-Yu Yu, Sarah Spitz, Francesca Michela Pramotton, Zhengpeng Wan, Ellen L Kan, Erin N Tevonian, Manuel Levy, Eva Lendaro, Roger D Kamm, Linda G Griffith, Fan Wang, Tong Qiu, Sixian You*. “Deep and Dynamic Metabolic and Structural Imaging in Living Tissues.” Science Advances, 2024, 10 (50), eadp2438 [Link] [PDF]

  5. Robert Jordan*, Stephanie L Ford-Scheimer, Rodolfo M Alarcon, Anthony Atala, Jeffrey T Borenstein, Kyle R Brimacombe, Sara Cherry, Hans Clevers, Mindy I Davis, Simon GP Funnell, Lee Gehrke, Linda G Griffith, Abigail C Grossman, Thomas Hartung, Donald E Ingber, Nicole C Kleinstreuer, Calvin J Kuo, Emily M Lee, Christine L Mummery, Thames E Pickett, Sasirekha Ramani, Edwin A Rosado-Olivieri, Evi B Struble, Zhengpeng Wan, Mark S Williams, Matthew D Hall, Marc Ferrer, Sarine Markossian*. “Report of the Assay Guidance Workshop on 3-Dimensional Tissue Models for Antiviral Drug Development.” The Journal of Infectious Diseases, 2023, jiad334. [Link] [PDF]

  6. Giovanni S Offeddu, Jean Carlos Serrano, Zhengpeng Wan, Mark A Bryniarski, Sara C Humphreys, Sophia W Chen, Hamsini Dhoolypala, Kip Conner, Roger D Kamm*. “Microphysiological endothelial models to characterize subcutaneous drug absorption.” ALTEX - Alternatives to animal experimentation. 2023, 40(2), pp. 299–313. doi: 10.14573/altex.2207131. [Link] [PDF]

  7. Shun Zhang#, Zhengpeng Wan#, Georgios Pavlou, Amy X Zhong, Liling Xu, Roger D Kamm*. “Interstitial Flow Promotes the Formation of Functional Microvascular Networks In Vitro through Upregulation of Matrix Metalloproteinase-2.” Advanced Functional Materials, 2022, 32, 2206767. [Link] [PDF]​

  8. Zhengpeng Wan, Amy X Zhong, Shun Zhang, Georgios Pavlou, Mark F Coughlin, Sarah E Shelton, Huu Tuan Nguyen, Jochen H Lorch, David A Barbie, Roger D Kamm*. “A Robust Method for Perfusable Microvascular Network Formation In Vitro.” Small Methods, 2022, smtd.202200143. [Link] [PDF]​

  9. Zhengpeng Wan#, Shun Zhang#, Amy X Zhong, Sarah E Shelton, Marco Campisi, Shriram K Sundararaman, Giovanni S Offeddu, Eunkyung Ko, Lina Ibrahim, Mark F Coughlin, Tiankun Liu, Jing Bai, David A Barbie*, Roger D Kamm*. “A robust vasculogenic microfluidic model using human immortalized endothelial cells and Thy1 positive fibroblasts.” Biomaterials, 2021, 276, 121032. [Link] [PDF]

  10. Shun Zhang, Zhengpeng Wan, Roger D Kamm*. “Vascularized organoids on a chip: Strategies for engineering organoids with functional vasculature.” Lab on a Chip, 2021, 21 (3), 473-488. [Link] [PDF]

  11. Jing Bai, Kristina Haase, Justine J Roberts, Joseph Hoffmann, Huu Tuan Nguyen, Zhengpeng Wan, Shun Zhang, Bapi Sarker, Nathan Friedman, Čedomila Ristić-Lehmann, Roger D Kamm*. “A novel 3D vascular assay for evaluating angiogenesis across porous membranes.” Biomaterials, 2021, 268, 120592. [Link] [PDF]

Neurological Disorders

  1. Tatsuya Osaki, Zhengpeng Wan, Koji Haratani, Ylliah Jin, Macro Campisi, David A Barbie, Roger D Kamm*, Mriganka Sur*. “miR126-mediated impaired vascular integrity in Rett syndrome.” bioRxiv 2024.10.11.617929. [Link] [PDF]

  2. Justin Rustenhoven*, Georgios Pavlou, Steffen E. Storck, Taitea Dykstra, Siling Du, Zhengpeng Wan, Daniel Quintero, Joshua P. Scallan, Igor Smirnov, Roger D. Kamm, Jonathan Kipnis*. “Age-related alterations in meningeal immunity drive impaired CNS lymphatic drainage.” Journal of Experimental Medicine 3 July 2023; 220 (7): e20221929. [Link] [PDF]

Synthetic Biology

  1. Lei Wang*, Wenlong Xu, Shun Zhang, Gregory C Gundberg, Christine R Zheng, Zhengpeng Wan, Kamila Mustafina, Fabio Caliendo, Hayden Sandt, Roger Kamm, Ron Weiss*. “Sensing and guiding cell-state transitions by using genetically encoded endoribonuclease-mediated microRNA sensors.” Nature Biomedical Engineering, 2024. [Link] [PDF]

Mechano-biology

  1. Yue Qiu, Qingyue Xiao, Yucai Wang, Yichen Cao, Jing Wang, Zhengpeng Wan, Xiangjun Chen, Wanli Liu, Li Ma, Chenguang Xu*. “Mechanical force determines chimeric antigen receptor microclustering and signaling.” Molecular Therapy, 2024, Volume 32, Issue 4, 1016 - 1032. [Link] [PDF]

  2. Zhengpeng Wan, Samina Shaheen, Alicia Chau, Yingyue Zeng, Wanli Liu*. “Imaging: gear up for mechano-immunology.” Cellular Immunology, 2020, 350, 103926. (Review) [Link] [PDF]

  3. Zhixun Shen, Sichen Liu, Xinxin Li, Zhengpeng Wan, Youxiang Mao, Chunlai Chen, Wanli Liu. “Conformational change within the extracellular domain of B cell receptor in B cell activation upon antigen binding.” eLife, 2019, eLife 2019;8:e42271. [Link] [PDF]

  4. Samina Shaheen, Zhengpeng Wan, Kabeer Haneef, Yingyue Zeng, Wang Jing, Wanli Liu*. “B cell mechanosensing: A mechanistic overview.” Advances in immunology, 2019, 144, 23-63. (Review) [Link] [PDF]

  5. Junyi Wang#, Feng Lin#, Zhengpeng Wan, Xiaolin Sun, Yun Lu, Jianyong Huang, Fei Wang, Yingyue Zeng, Ying-Hua Chen, Yan Shi, Wenjie Zheng, Zhanguo Li, Chunyang Xiong* and Wanli Liu* “Profiling the origin, dynamics, and function of traction force in B cell activation.” Science Signaling, 2018, Vol. 11, Issue 542, eaai9192. [Link] [PDF]

  6. Zhengpeng Wan, Chenguang Xu#, Xiangjun Chen#, Hengyi Xie#, Zongyu Li#, Jing Wang#, Xingyu Ji, Haodong Chen, Qinghua Ji, Samina Shaheen, Fei Wang, Jizhong Lou, Zhuo Tang, Wanli Liu*. “PI(4,5)P2 determines the threshold of mechanical force–induced B cell activation.” Journal of Cell Biology, 2018, jcb.201711055. [Link] [PDF]

  7. Samina Shaheen#, Zhengpeng Wan#, Zongyu Li#, Alicia Chau, Xinxin Li, Shaosen Zhang, Yang Liu, Junyang Yi, Yingyue Zeng, Jing Wang, Xiangjun Chen, Liling Xu, Wei Chen, Fei Wang, Yun Lu, Wenjie Zheng, Xiaolin Sun, Zhanguo Li, Chunyang Xiong, Wanli Liu*. “Substrate stiffness governs the initiation of B cell activation by the concerted signaling of PKCβ and focal adhesion kinase.” eLife, 2017, eLife 2017;6:e23060. [Link] [PDF]​

  8. Zhengpeng Wan, Xiangjun Chen, Haodong Chen, Qinghua Ji, Yingjia Chen, Jing Wang, Yiyun Cao, Fei Wang, Jizhong Lou, Zhuo Tang, and Wanli Liu*. “The activation of IgM- or isotype-switched IgG- and IgE-BCR exhibits distinct mechanical force sensitivity and threshold.” eLife, 2015; 4:e06925. (Faculty of 1000 recommended paper) [Link] [PDF]

  9. Yingyue Zeng, Junyang Yi, Zhengpeng Wan, Kai Liu, Ping Song, Alicia Chau, Fei Wang, Zai Chang, Weidong Han, Wenjie Zheng, Ying-Hua Chen, Chunyang Xiong, and Wanli Liu*. “Substrate stiffness regulates B-cell activation, proliferation, class switch and T-cell-independent antibody responses in vivo.” European Journal of Immunology, 2015, 45:1621-1634. [Link] [PDF]

  10. Zhengpeng Wan, Shaosen Zhang, Yilin Fan, Kai Liu, Feng Du, Angel M. Davey, Huiyuan Zhang, Weidong Han, Chunyang Xiong, and Wanli Liu*. “B cell activation is regulated by the stiffness properties of the substrate presenting the antigens.” The Journal of Immunology, 2013, 190(9):4661-75. (Faculty of 1000 recommended paper) [Link] [PDF]

  11. Zhengpeng Wan, Wanli Liu*. “The growth of B cell receptor microcluster is a universal response of B cells encountering antigens with different motion features.” Protein & Cell, 2012, 3(7): 545-558. [Link] [PDF]

Immunology
Autoimmune
B cell activation

  1. Yu Wang#, Shaocun Zhang#, *, Na Kang, Lihui Dong, Haochen Ni, Sichen Liu, Siankang Chong, Zhengli Ji, Zhengpeng Wan, Xiangjun Chen, Fei Wang, Yun Lu, Baidong Hou, Pei Tong, Hai Qi, Meng Michelle Xu*, Wanli Liu*. “Progressive polyadenylation and m6A modification of Ighg1 mRNA maintain IgG1 antibody homeostasis in antibody-secreting cells” Immunity, 2024. 10.1016/j.immuni.2024.10.004. [Link] [PDF]

  2. Yu Wang, Zhixun Shen, Zhengpeng Wan*, Wanli Liu*. “Site-specific Labeling of B Cell Receptor and Soluble Immunoglobulin.” Bio-protocol, 2020, 10 (18), e3767-e3767. [Link] [PDF]

  3. Chenguang Xu, Zhengpeng Wan, Samina Shaheen, Jing Wang, Zhiyong Yang, Wanli Liu*. “A PI (4, 5) P2‐derived “gasoline engine model” for the sustained B cell receptor activation.” Immunological Reviews, 2019, 291 (1), 75-90. (Review) [Link] [PDF]

  4. Jing Wang, Zhengpeng Wan, Wanli Liu*. Understanding of B Cell Receptor Signaling Through a Photo-Activatable Antigen Presentation System. In: Liu C. (eds) B Cell Receptor Signaling. Methods in Molecular Biology, 2018, vol 1707. Humana Press, New York, NY. (Book) [Link] [PDF]

  5. Shan Tang#, Zhengpeng Wan#, Yiren Gao#, Ji-Shen Zheng, Jing Wang, Yan-Yan Si, Xin Chen, Hai Qi, Lei Liu* and Wanli Liu*. “Total chemical synthesis of photoactivatable proteins for light-controlled manipulation of antigen–antibody interactions.” Chemical Science, 2016, 7, 1891-1895. [Link] [PDF]

  6. Jing Wang#, Shan Tang#, Zhengpeng Wan, Yiren Gao, Yiyun Cao, Junyang Yi, Yanyan Si, Haowen Zhang, Lei Liu* and Wanli Liu*. “Utilization of a photoactivatable antigen system to examine B cell probing termination and the B cell receptor sorting mechanisms during B cell activation.” Proc. Natl. Acad. Sci. USA. 2016, doi:10.1073/pnas.1517612113. [Link] [PDF]

  7. Xiangjun Chen, Gen Li, Zhengpeng Wan, Ce Liu, Yingyue Zeng, and Wanli Liu*. “How B cells remember? A sophisticated cytoplasmic tail of mIgG is pivotal for the enhanced transmembrane signaling of IgG-switched memory B cells.” Progress in Biophysics and Molecular Biology. 2015, 118:89-94. (Review) [Link] [PDF]

  8. Liling Xu, Gen Li, Jing Wang, Yilin Fan, Zhengpeng Wan, Shaosen Zhang, Samina Shaheen, Jing Li, Li Wang, Cai Yue, Yan Zhao, Fei Wang, Joseph Brzostowski, Yinghua Chen*, Wenjie Zheng*, Wanli Liu*. “Through an ITIM-Independent Mechanism the FcγRIIB Blocks B Cell Activation by Disrupting the Colocalized Microclustering of the B Cell Receptor and CD19.” The Journal of Immunology 192, no. 11 (2014): 5179-5191. (Faculty of 1000 recommended paper) [Link] [PDF]

  9. Shaosen Zhang, Liling Xu, Xingwang Zhao, Xin Chen, Yilin Fan, Zhengpeng Wan, Yinsheng Xu, and Wanli Liu*. “A New and Robust Method of Tethering IgG Surrogate Antigens on Lipid Bilayer Membranes to Facilitate the TIRFM Based Live Cell and Single Molecule Imaging Experiments.” PloS one 8, no. 5 (2013): e63735. [Link] [PDF]

  10. Wanli Liu, Elizabeth Chen#, Xingwang Zhao#, Zhengpeng Wan#, Yiren Gao#, Angel Davey#, Eric Huang, Lijia Zhang, Jillian Crocetti, Gabriel Sandoval, M. Gordon Joyce, Carrie Miceli, Jan Lukszo, L. Aravind, Wojciech Swat, Joseph Brzostowski, and Susan K. Pierce*. “The scaffolding protein synapse associated protein 97 is required for enhanced signaling through isotype-switched IgG memory B cell receptors.” Science Signaling 2012, 5(235): ra54. [Link] [PDF]

Pending Patents

  1. Fluidic Platforms for Perfusable Vascularized Tissues. Roger Kamm, Zhengpeng Wan, Shun Zhang. US Patent App. 17/728,642

Contact
Information

Department of Biomedical Engineering
Michigan Technological University

H-STEM 333

1400 Townsend Dr

Houghton MI 49931

wanzp at mtu.edu

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